DSpace Coleção:
http://repositorio.unifap.br:80/jspui/handle/123456789/85
2024-02-29T02:53:40ZAtividade antibiofilme de nanopartículas poliméricas carregadas com ácido anacárdio e seu efeito desmineralizante em esmalte bovino
http://repositorio.unifap.br:80/jspui/handle/123456789/361
Título: Atividade antibiofilme de nanopartículas poliméricas carregadas com ácido anacárdio e seu efeito desmineralizante em esmalte bovino
Abstract: Introduction: Caries represents a public health problem, which despite the prophylactic methods, is still very prevalent. It can be triggered by several factors, but its formation is dependente on the bacterial biofilm. Objective: To evaluate the anticaries prophilatic activity and the demineralization associated with anacardic acid loaded zein nanoparticles in bovine enamel slabs. Methodology: Standard cavities were made in 36 slabs, restored with conventional glass ionomer cement subjected to different treatments, while 12 sound slabs, without cavities were used as controls, 6 cleansed with the test nanoparticles and 6 with sterile saline solution. The slabs were submitted to Streptococcus mutans biofilm formation assay in BHI sterile broth supplemented with 10% glucose, cultured in microanaerobiosis. The antimicrobial effect was assessed by counting the CFU per mg of biofilm dried weight. The microhardness loss (ΔS) was measured at distances of 50 μm and 150 μm at different depths. The results were submitted to analysis of variance (ANOVA) with Tukey post-test, assuming a confidence level of 5%.Results and discussion: Although the CHX and AaNp-L-GIC groups were prominently superior to their pairs, no significant difference was found in the biofilm inhibition within the groups. However, the groups treated with anacardic acid nanoparticles presented a notorious reduction in the mean growth in comparasion to the other groups, demonstrating its antimicrobial effect. Regarding the loss of hardness, BNp-C-GIC group differed statistically from some groups, presenting the highest demineralization, what could be related to the nutrients removal from the biofilm. Conclusions: Our findings indicate that the use of polymeric anacardic acid nanoparticles, incorporated into the GIC or as cleanser, could be a prophylactic option against the S. mutans biofilm in both restored and unrestored enamel and as such could be tested as a potential anticaries agent0026-11-08T00:00:00ZSoroprevalência do vírus da Hepatite B (HVB) no município de Macapá, Amapá, Brasil
http://repositorio.unifap.br:80/jspui/handle/123456789/360
Título: Soroprevalência do vírus da Hepatite B (HVB) no município de Macapá, Amapá, Brasil
Abstract: Introduction: Viral hepatitis are diseases triggered by viruses with tropism by hepatocytes, presenting similar signs and symptoms, but with differences related to possible means of contamination and degree of infection. It is currently considered a serious public health problem due to the associated morbidity, with evolution to hetocellular carcinoma, which is the ninth leading cause of death on the world stage.Objective: The objective was to determine the prevalence and to establish the epidemiological profile of viral hepatitis B in the municipalities of Macapá, State of Amapá. Methodology: This is a qualitative-quantitative study. All participants in this study answered a standardized questionnaire containing questions related to personal identification, family composition and habits and practices that may be related to the risk of infection by hepatitis B virus and submitted to rapid test for HBV and determination of antibodies against the virus surface antigen of hepatitis B virus. Results and discussions: We identified the main risk foams that the study is exposed to, as a means of contamination, familar conviviality (sharing of personal objects) and sexual counts unprotected. In the rapid tests, no sample was reactive for the presence of the virus. In the assay for antibody determination 65.17% of the samples were positive for the presence of antibodies, from 12.36% presented concentration below 20 mIU/mL, confirming, according to clinical practice the presence of the antibody, but not necessarily immunity. Conclusions: These results serve as a basis to assist in the elaboration of campaigns or strategies for prevention and control of these infections that are more adequate for each regional health and in the basic planning strategies of the STD/AIDS state program and HEPATITES VIRES0022-01-08T00:00:00ZEstudo do óleo essencial e da nanoemulsão à base de Rosmarinus officinalis L. sobre dislipidemia em ratos Wistar
http://repositorio.unifap.br:80/jspui/handle/123456789/359
Título: Estudo do óleo essencial e da nanoemulsão à base de Rosmarinus officinalis L. sobre dislipidemia em ratos Wistar
Abstract: Introduction: Dyslipidemias are metabolic alterations of lipids which induce increased serum lipoproteins, cholesterol, and triglycerides, being a risk factor for the development of atherosclerosis.Objective: This study aimed to assess the effect of Rosmarinus officinalis’ essential oil (ORO), and its nanoemulsion (NEORO) on dyslipidemia induced by Triton and Cocos nucifera saturated fat (GSC) in Wistar rats. Methodology: The induction of dyslipidemia was performed in two models: in the first, 2 mL of GSC were administered orally, while in the second induction model Triton was used at a dose of 150 mg / kg intraperitoneally, in both animals the animals were treated with ORO (100 mg / kg) and NEORO (500 μg / kg).Then, blood samples were collected for biochemical analysis; after animals were euthanized, their organs and abdominal fat were removed for analysis, and the aorta was assessed through MEV for atheromatous processes. Statistical analysis was performed using the software GraphPad Prism (7.0), values were represented as a mean and standard deviation. Results and Discussion: In the phytochemical evaluation of ORO, the major compounds detected by gas chromatography coupled to mass spectrometry (GC-MS) shows the presence of α-pinene (8.13%), limonene (21.99%), 1,8-cineole (33.70%), and camphor (27.68%). Triton-induced dyslipidemia led to a significant increase of total cholesterol, LDL, and triglycerides. Groups treated with ORO and NEORO had a significant decrease in total cholesterol, LDL, and triglycerides, just as the group treated with simvastatin (SIN). GSC-induced dyslipidemia resulted in an increased amount of abdominal fat, hypercholesterolemia, hyperglyceridemia, increased levels of LDL, and atheromatous process formation in the aorta. All treated groups (ORO+GSC, NEORO+GSC, SIN+GSC) had a significant decrease of hypercholesterolemia, hyperglyceridemia, abdominal fat, and inhibition of atheromatous process formation in the vascular endothelium.Conclusion: The results reported in this study evidence that treatment either with ORO or NEORO exerts anti-dyslipidemic and anti-atherogenic effects in the tested models0020-06-09T00:00:00ZAvaliação da atividade larvicida do extrato bruto etanólico e óleos essenciais das folhas de origanum majorana L. e origanum vulgare L. frente ao Aedes aeggypti (Linnaeus, 1762)
http://repositorio.unifap.br:80/jspui/handle/123456789/358
Título: Avaliação da atividade larvicida do extrato bruto etanólico e óleos essenciais das folhas de origanum majorana L. e origanum vulgare L. frente ao Aedes aeggypti (Linnaeus, 1762)
Abstract: Introduction: Origanum majorana L. and Origanum vulgare L. belong to the family Lamiaceae and are popularly known as manjorana and oregano, respectively. Objective: To evaluate the larvicidal activity of O. majorana L. and O. vulgare L. against Aedes aegypti. Methodology: the larvicidal bioassay was performed with larvae in the third stage of A. aegypti; the identifications of the components of the oils by Gas Chromatography Coupled to the Mass Spectrophotometer; the crude ethanolic extract by phytochemical evaluation; the microbiological activity by the microdilution method with bacteria Pseudomona aeruginosa, Escherichia coli and Staphylococcus aureus; the antioxidant was evaluated by the sequestration method of the 2.2-diphenyl-1-picryl-hydrazyl radical and the cytotoxic activity was performed using Artemia saline. Results and discussion: Manjerona and oregano oils presented high larvicidal activity with LC50 of 74.63 and 56.00 μg.mL-1 after 24h, LC50 of 20.27 and 15.69 μg.mL-1 respectively, after 48h. Manjerona extract showed good activity with LC50 of 219.14 and 79.40 μg.mL-1 after 24h and 48h, respectively. The major constituents found in the manjerona and oregano oils were pulegone (57.05%), γ-terpinene (27.18%), respectively. In the extract were found reducing sugars, tannins, phenols, depsides and depsedones, steroids and triterpenoids. P. aeruginosa and E. coli were more susceptible in oils and extract with MIC of 31.25 μg.mL-1. Only the oil of oregano opposite S. aureus with MIC of 500 μg.mL-1. In the MBM of oregano oil, concentrations of 250, 500 and 1000 μg.mL-1 inhibited the growth of P. aeruginosa, S. aureus and E. coli, respectively. In manjerona oil the concentrations of 1000 and 500 μg.mL-1 inhibited the growth of P. aeruginosa and E. coli. There was no antioxidant activity of the plants, but there was cytotoxic activity of oregano and manjerona oils at A. salina with high LC50 of 38.11 and 172.6 μg.mL-1 and low LC50 of 946.9 μg.mL-1 extract. Conclusions: In this way, the oils and extract obtained from the leaves of O. vulgare L. and O. majorana L. present potential for the development of natural larvicides0031-10-10T00:00:00Z