Obtenção do óleo da Astrocaryum aculeatum (tucumã) e sua avaliação genotóxica e/ou antigenotóxica in vivo com tratamento agudo em camundongo swiss

Abstract

Astrocaryum aculeatum (Tucumã), is a native Amazon fruit rich in carotenoids and polyphenols. In this way, the present study aimed to obtain the fixed oil of Astrocaryum aculeatum and its genotoxic and/or antigenotoxic evaluation in vivo with acute treatment in Swiss mouse. The methodology was used, the fruit was dried, peeled and stored in a greenhouse for 50º per 24h and cooled by room temperature. The resulting mass was 28.21% of the total of the fruit, being subjected to hydraulic pressing, obtaining 22, 21% of the oil. Animals (males - 6 per group) were used to gavage the oil at concentrations of 500, 1000 and 2000 mg / kg b.p. (Genotoxic groups); And Doxorubicin-DXR-associated intraperitoneally at the concentration of 15 mg / kg b.p. (Antigenotoxic groups). Dimethylsufoxide-DMSO, the solvent used, was evaluated in isolation to exclude possible influence on the results. All groups were analyzed from the laminae from smears with peripheral blood, which was collected 24h and 48h after the appropriate treatments, where 2000 cells per animal were analyzed. Results and Discussion: The genotoxic groups did not show statistically significant differences in relation to the negative control, and in relation to the antigenotoxic test there was a statistically significant difference for all the concentrations used in relation to the positive control group (DXR), with a significant reduction of about 36 , 57 ± 1.74% for treatments of 24h and 65,18 ± 1,29% for those of 48h, in the frequency of PCEMNs. Conclusion: The AA presented a relevant antigenotoxic effect, and no genotoxic effect, for all the concentrations used in both 24h and 48h