Avaliação do efeito do Diazinon na composição e sucessão de assembleias de calliphoridae em carcaças de coelhos domésticos

Abstract

The Forensic Entomotoxicology consists of detection and analysis of the toxic substances interference on the biology of necrophagous insects. The fatal poisonings caused by diazinon could happen accidentally or after a suicide attempt. The goal of this study was to evaluate the effect of the diazinon present in the carcasses of domestic rabbits on the composition and succession of Calliphoridae. Nine carcasses of male rabbits were utilized, weighing, approximately, 2 Kg each that were divided into three groups, each group with three replicas, namely: a control group, and two treatment groups, T1 100 mg/Kg and T2 300 mg/Kg. Three fragments of Amazonian Cerrado were selected. In each of them, a portion of 100m x 50m was defined, where three carcasses where placed (one from each group) with a distance of 25m from each other. Five stages of decomposition were observed, Fresh, Chromatic-Bloat, Black Putrefaction and Skeletonization. Eight Calliphoridae species in the adult phase were collected, Chloroprocta idiodea (0.1 %), Chrysomya albiceps (58.3 %), Chrysomya megacephala (14.2 %), Chrysomya putoria (2.6 %), Cochliomyia hominivorax (1.3 %), Cochliomyia macellaria (0.5 %), Lucilia eximia (19.8 %) and Paralucilia paraensis (3.3 %). The greatest abundance of adult specimens in the Control group only started in the Black Putrefaction phase. The Skeletonization phase was more abundant in the control carcasses when compared to the carcasses of the treated groups. A total of 941 Calliphoridae individuals were collected in the immature stage, belonging to three species. C. albiceps (76.3 %) and C. putoria (1 %) and L. eximia (22,7). The carcasses of the T2 treatment were the ones that presented the lowest number of immature colonizers. The number of immature individuals collected on the control carcasses was higher when compared to the treatments (p= 0.00469). The diazinon present in carcasses of domestic rabbits interferes on their putrefaction time, retarding the decomposition phases and influences the colonization by immature forms of Calliphoridae