Investigação molecular do mecanismo de ação antidiabética da nanodispersão de uma fração flavonoídica de Baccharis reticularia

Abstract

Introduction: Diabetes mellitus is a group of metabolic diseases that changes primarily the homeostasis of carbohydrate metabolism followed by changes in lipidic and protein metabolisms. It is characterized by insulin action inefficacy as a consequence of its reduced production and/or its tissue resistance, causing hyperglycemia in fasting. In a previous study evaluating a nanodispersion of flavonoic fraction isolated from Baccharis reticularia DC. applied in aloxan-diabetic rats it was possible to show the efficiency of this treatment in reducing the diabetes. Objective: The aim of this work is to evaluate changes in gene expression by Real Time Quantitative Polymerase Chain Reaction (RT-qPCR) as a way of elucidate the mechanism of action from this nanodispersion in glucose`s reducing in aloxandiabetic rats. Methods: We have chose some enzymes related to glucose metabolism, of the insulin and sodium-glucose cotransporter as targets in liver, pancreas and kidney respectively. Results: It was possible to quantify all the targets used, however we could not find any significant change in gene expression of the selected targets. Conclusion: We suggest the investigation of another targets in a future study as a way of elucidate the biological function of flavonoic nanodispersion from B. reticularia in reducing diabetes in rats